Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135052_z8zb CTCCACTTCCCATCTGACCGCTGATGTGCTGG(+AAAAAA)GATCCCAGAGCATACTCCTGAGCCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606135050_94bx GTCATAAAGCCAGGCTCAGGAGTATGCTCTGG(+AAAAAA)GATCCCAGCACATCAGCGGTCAGATGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606135049_nqlo TATGTTAATATGATGAAATAAAATCAGAACTG(+AAAAAA)GTACCTATTTGTACGTAAGTGTGAGACGTGG NGG 10 8 17 8 24 0 100 5 10
    240606135048_c5ew AGAAGCCACGTCTCACACTTACGTACAAATAG(+AAAAAA)GTACCAGTTCTGATTTTATTTCATCATATTA NGG 10 8 17 8 24 0 100 5 10
    240606135047_w9h8 TTCAGTTTCCAGTGACCCACAAAAGGGGCGTG(+AAAAAA)GTACCGGAAAAACAAAAAGATGGTGTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606135045_s562 CCCTGCCAGAACACCATCTTTTTGTTTTTCCG(+AAAAAA)GTACCACGCCCCTTTTGTGGGTCACTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606135044_v7xc GTATTTGGCTTAATCTCCCCATTTGATATAAG(+AAAAAA)GTACCAGTCATTTTTAGAGGCCAAAAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606135043_wyt4 GGGTTCCTTTTTTTGGCCTCTAAAAATGACTG(+AAAAAA)GTACCTTATATCAAATGGGGAGATTAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606135042_iqi9 ACAGTGAATGGGCCAAACTGTGTTTATGGTTG(+AAAAAA)GATCCCGAACAGTGTGAAAAGACAAGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606135040_djjb AATGACCACTCTTGTCTTTTCACACTGTTCGG(+AAAAAA)GATCCAACCATAAACACAGTTTGGCCCATTC NGG 10 8 17 8 24 0 100 5 10