Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135106_4osl CGCTTTTTCTTCTTCTTTTAATTTCTCTATGG(+AAAAAA)GATCCTGGAGTTCTGGTTCCTTACTGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606135105_sqac GGGTTGCCTTCAGTAAGGAACCAGAACTCCAG(+AAAAAA)GATCCCATAGAGAAATTAAAAGAAGAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606135103_s2t3 AAATAACCTAGAATCATCTCCAGACTCTAAAG(+AAAAAA)GATCCTATTCATTGTTTCCCCCTCCCCAAGG NGG 10 8 17 8 24 0 100 5 10
    240606135102_grst GTTGACCTTGGGGAGGGGGAAACAATGAATAG(+AAAAAA)GATCCTTTAGAGTCTGGAGATGATTCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606135100_824n AAGTGACTCTTCGGGTGGCTTTCCCTCTCAGG(+AAAAAA)GTACCTAGAGGTGTGAATTTGAAGCCATGCT NGG 10 8 17 8 24 0 100 5 10
    240606135059_ww15 TTCCCAGCATGGCTTCAAATTCACACCTCTAG(+AAAAAA)GTACCCTGAGAGGGAAAGCCACCCGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606135058_ip2q TATTCACACTCCTAATCAAACGTGCACTATGG(+AAAAAA)GTACCTAGTTTTTCTCTTAAGAACATACATG NGG 10 8 17 8 24 0 100 5 10
    240606135056_e5ok ACTTACATGTATGTTCTTAAGAGAAAAACTAG(+AAAAAA)GTACCCATAGTGCACGTTTGATTAGGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606135055_juq7 TTATTCAAATTTTGATTATTTTAAAAATAAAG(+AAAAAA)GATCCATTCTTACCGAAAGGGACACTACATC NGG 10 8 17 8 24 0 100 5 10
    240606135054_5q0u TTAAAGATGTAGTGTCCCTTTCGGTAAGAATG(+AAAAAA)GATCCTTTATTTTTAAAATAATCAAAATTTG NGG 10 8 17 8 24 0 100 5 10