Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135204_h64c TATGTAGAATATACTGGTCTCCAACTCACAAG(+AAAAAA)GATCCACCTGCCTCTGCCTCCTGAGGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606135203_lfjd CAGTCTCACCCCTCAGGAGGCAGAGGCAGGTG(+AAAAAA)GATCCTTGTGAGTTGGAGACCAGTATATTCT NGG 10 8 17 8 24 0 100 5 10
    240606135201_oa42 TGAGCTGAGCGTCTCTATCCTTCCCTGTTCAG(+AAAAAA)GATCCTTCCTGCAGGTGCTCATGAGATAGTA NGG 10 8 17 8 24 0 100 5 10
    240606135200_fn6v GATGCTACTATCTCATGAGCACCTGCAGGAAG(+AAAAAA)GATCCTGAACAGGGAAGGATAGAGACGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606135158_z6ll CCAGTCTACACAGACAGGCCCCATGGATTCTG(+AAAAAA)GTACCTTCAGTTATGGGAGTTATTTGTAATT NGG 10 8 17 8 24 0 100 5 10
    240606135157_r7m9 ACACAAATTACAAATAACTCCCATAACTGAAG(+AAAAAA)GTACCAGAATCCATGGGGCCTGTCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606135156_xvlv CTAGGCAGCTGTATAGAGAAAACTCAGAGGTG(+AAAAAA)GTACCTGGTTTCCCATGTGGGTCTAAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606135154_8ltg CCAAGACTGCTTAGACCCACATGGGAAACCAG(+AAAAAA)GTACCACCTCTGAGTTTTCTCTATACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606135152_jcs2 TTTCCTGTCATCAAGAAGCTATCACATTTTTG(+AAAAAA)GTACCTCTATAAAGCTAACCCTTCACAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606135151_7ru1 CTTTTCTTTTGTGAAGGGTTAGCTTTATAGAG(+AAAAAA)GTACCAAAAATGTGATAGCTTCTTGATGACA NGG 10 8 17 8 24 0 100 5 10