Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135136_uz3c ACCAAATATACAGCATCAATAAAATGTACACG(+AAAAAA)GTACCATGAAAAATTCGAGAACTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606135135_w77o GAAAAGCAGCTTGAGTTCTCGAATTTTTCATG(+AAAAAA)GTACCGTGTACATTTTATTGATGCTGTATAT NGG 10 8 17 8 24 0 100 5 10
    240606135134_s7k2 ACTGCTGGTGTATCTGTGAAGTGTTTGCAGTG(+AAAAAA)GATCCATCTGCCGCTGCCTGCTAACATATGA NGG 10 8 17 8 24 0 100 5 10
    240606135133_xm9v TTAGTTCATATGTTAGCAGGCAGCGGCAGATG(+AAAAAA)GATCCACTGCAAACACTTCACAGATACACCA NGG 10 8 17 8 24 0 100 5 10
    240606135131_g25p GTTCCAGGAGCCTCCTCATGTTTGCCTGCTGG(+AAAAAA)GATCCCTGCACAAGGTGTTTCTTTACAATAG NGG 10 8 17 8 24 0 100 5 10
    240606135130_dda2 CTGGTCTATTGTAAAGAAACACCTTGTGCAGG(+AAAAAA)GATCCCAGCAGGCAAACATGAGGAGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606135128_6zij AATACTACCATAAATAACTAGCACCCACCAAG(+AAAAAA)GATCCCAAGTTCCTGTCTTTTCCAAATGGCT NGG 10 8 17 8 24 0 100 5 10
    240606135126_0zk7 ACCCTAGCCATTTGGAAAAGACAGGAACTTGG(+AAAAAA)GATCCTTGGTGGGTGCTAGTTATTTATGGTA NGG 10 8 17 8 24 0 100 5 10
    240606135125_3r3j GTCAGGAGAACACTTGAAAGGCTTTCTTTGTG(+AAAAAA)GTACCTACGGGGTGAAATCACAGCCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606135123_axx8 CTCTCTTGACAGGCTGTGATTTCACCCCGTAG(+AAAAAA)GTACCACAAAGAAAGCCTTTCAAGTGTTCTC NGG 10 8 17 8 24 0 100 5 10