Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133806_ygzs GGTTTATGATGAAAACTTTATTCTGCATCAGG(+AAAAAA)GTACCTGGAAAGTGAAGCTGCATTTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606133803_0jn3 CCTGCATTACCTGCCAGCGAGTCAAGGTAGGG(+AAAAAA)GATCCAGCAAACGGTTTTGGGAATTCAGACT NGG 10 8 17 8 24 0 100 5 10
    240606133802_yc8x TCCACAGTCTGAATTCCCAAAACCGTTTGCTG(+AAAAAA)GATCCCCTACCTTGACTCGCTGGCAGGTAAT NGG 10 8 17 8 24 0 100 5 10
    240606133800_aovm TGAAGCACTCTGCATCCTCAGGGGAACTTGGG(+AAAAAA)GTACCCAATGGAAACATTTGTAAAAGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240606133759_eam7 ATTTAATGAACTTTTACAAATGTTTCCATTGG(+AAAAAA)GTACCCCAAGTTCCCCTGAGGATGCAGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606133757_wvyj GCTGTCTTCAGACACACCAGAAGAGGGCATTG(+AAAAAA)GATCCCATTACAGATGGTTGTAAACCACTAT NGG 10 8 17 8 24 0 100 5 10
    240606133756_77ho ACCACATAGTGGTTTACAACCATCTGTAATGG(+AAAAAA)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606133754_0x99 GGTGGAATTATGATGGAGTTCTGACTTTATTG(+AAAAAA)GTACCATGAAAGTCAGGGTATACTTGACTAA NGG 10 8 17 8 24 0 100 5 10
    240606133753_ovjz ATACTTTAGTCAAGTATACCCTGACTTTCATG(+AAAAAA)GTACCAATAAAGTCAGAACTCCATCATAATT NGG 10 8 17 8 24 0 100 5 10
    240606133752_mtbx CAGCCTTCCAAGTCATGAACCTTCTTTGAGAG(+AAAAAA)GATCCACCCCACTAGTCCTTCCCCCTCGCAC NGG 10 8 17 8 24 0 100 5 10