Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133732_dzyq CACTACTATAGTGGAGTTACCACCTTGGACAG(+AAAAAA)GTACCACAGATGGCTCAAAACAGGAAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606133730_nwy6 TGGACTGTCCACTAGAGTTGGACAGTACTGGG(+AAAAAA)GTACCCAGGTCCACCCCTTGGAATACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606133728_p733 TCCAGACAGGGTATTCCAAGGGGTGGACCTGG(+AAAAAA)GTACCCCAGTACTGTCCAACTCTAGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240606133727_sfw2 TAGCAAGCTCACTCACCAAGCCGCACTGGGTG(+AAAAAA)GATCCATCTTTGGTTTGTTGGAGAGAACAGA NGG 10 8 17 8 24 0 100 5 10
    240606133726_ehur TAGAGTCTGTTCTCTCCAACAAACCAAAGATG(+AAAAAA)GATCCACCCAGTGCGGCTTGGTGAGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606133724_hbow TTGTTTCCTCCCAAATACTCCCTGAACGGGAG(+AAAAAA)GATCCCCTTCTGCTCCATCAGGACTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240606133722_pzc7 GGCTTCAGTGGAGTCCTGATGGAGCAGAAGGG(+AAAAAA)GATCCTCCCGTTCAGGGAGTATTTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606133721_mxtr CCCACCACGCTCCCTAGCTGGGAGAGAAACAG(+AAAAAA)GATCCACAAGAGGAGTGGGAAAGCCGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606133720_b9b7 GTGTCAAAAACGGCTTTCCCACTCCTCTTGTG(+AAAAAA)GATCCTGTTTCTCTCCCAGCTAGGGAGCGTG NGG 10 8 17 8 24 0 100 5 10
    240606133717_bwda TAGGCTATATACCATGTTTGCAGTCAGCCTGG(+AAAAAA)GATCCATGAAACCCTGTCTCAAGAAAAGCAA NGG 10 8 17 8 24 0 100 5 10