Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175054_0fwa AAAAGCCTGTTTTCACAATACCAGCAGAGCAG(+AGGACA)GTACCCACATACATCCTCCTGCAGACGGGAG NGG 10 8 17 8 24 0 100 5 10
    240613175052_m7z4 GTGAACTCCCGTCTGCAGGAGGATGTATGTGG(+AGGACA)GTACCTGCTCTGCTGGTATTGTGAAAACAGG NGG 10 8 17 8 24 0 100 5 10
    240613175050_2fh7 TTTCTCCATACACACATTTATATTTAGAGAGG(+AGGACA)GATCCAAACCAGACATGCTGATGTTGGATCT NGG 10 8 17 8 24 0 100 5 10
    240613175049_epf6 ACCTTAGATCCAACATCAGCATGTCTGGTTTG(+AGGACA)GATCCCTCTCTAAATATAAATGTGTGTATGG NGG 10 8 17 8 24 0 100 5 10
    240613175047_b9ol GTGACATGTACAATATATAGAGGCCCCCACAG(+AGGACA)GATCCCCTGCTCCTGGACCTGAGCCCAACCA NGG 10 8 17 8 24 0 100 5 10
    240613175045_e6wc GCACCTGGTTGGGCTCAGGTCCAGGAGCAGGG(+AGGACA)GATCCTGTGGGGGCCTCTATATATTGTACAT NGG 10 8 17 8 24 0 100 5 10
    240613175043_c548 TGACCCCAGGAGACCAGAAAGATTGGGCTGGG(+AGGACA)GATCCAGAACCCACTCCTTAATCCTCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613175041_8is5 TGGATCAACTGAGGATTAAGGAGTGGGTTCTG(+AGGACA)GATCCCCAGCCCAATCTTTCTGGTCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613175040_6uu8 GCTTTGTGAAGTTGCTGTTAATAGGCCTTTGG(+AGGACA)GTACCCTTTGCTTGAAGTTATATCAACTGAG NGG 10 8 17 8 24 0 100 5 10
    240613175038_0ces ATCACCTCAGTTGATATAACTTCAAGCAAAGG(+AGGACA)GTACCCAAAGGCCTATTAACAGCAACTTCAC NGG 10 8 17 8 24 0 100 5 10