Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175036_11ba CCATATATGTAAGTGCCCCGTGTGTGTGCCTG(+AGGACA)GTACCGACAGAAGCCAAATGAGTATGTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613175035_3zlo GGAACTCTGACATACTCATTTGGCTTCTGTCG(+AGGACA)GTACCAGGCACACACACGGGGCACTTACATA NGG 10 8 17 8 24 0 100 5 10
    240613175033_9d0h GGCCCATGGAGACCGGGCCATGGAGAGCCTTG(+AGGACA)GTACCCGGATCGGTATACTCAGGCCATTGAA NGG 10 8 17 8 24 0 100 5 10
    240613175031_clnt GAGTGTTCAATGGCCTGAGTATACCGATCCGG(+AGGACA)GTACCAAGGCTCTCCATGGCCCGGTCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613175029_79ly TCCCTTCCTCGGTATTGTCACCTATATGCCAG(+AGGACA)GATCCCCTAGGACTTCCAAATTTCTTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613175027_0iqh GTCAGGTCTCAAGAAATTTGGAAGTCCTAGGG(+AGGACA)GATCCTGGCATATAGGTGACAATACCGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613175025_mpfz GGTGAGAAGGGTGTGGCTCGGATGGCATCCTG(+AGGACA)GTACCCACAGTGAGCTGGTAGAATACTAAGT NGG 10 8 17 8 24 0 100 5 10
    240613175023_b1vi AGATTACTTAGTATTCTACCAGCTCACTGTGG(+AGGACA)GTACCAGGATGCCATCCGAGCCACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613175022_wovr GAAAGGCCCAGTGTGGTACCAGACACACAGTG(+AGGACA)GATCCTTCCAGAGGTGCAGGTCTGTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240613175020_aq74 ACCACTAATAAACAGACCTGCACCTCTGGAAG(+AGGACA)GATCCACTGTGTGTCTGGTACCACACTGGGC NGG 10 8 17 8 24 0 100 5 10