Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131121_f35u CTGTGAACCAGCTATTCCGGTGTCATAGCTGG(+AAAAAA)GTACCTAACTTACTTCCATATGTGAAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606131120_wn8l TTTAGCACACTTCACATATGGAAGTAAGTTAG(+AAAAAA)GTACCCAGCTATGACACCGGAATAGCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606131119_k22h ACATTCATCTTGGTTTTCCTTCTTTATCACAG(+AAAAAA)GATCCAGCCTGGTGTTTCATGTATAGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240606131117_hrev TCAGTGCCTACTATACATGAAACACCAGGCTG(+AAAAAA)GATCCTGTGATAAAGAAGGAAAACCAAGATG NGG 10 8 17 8 24 0 100 5 10
    240606131116_vqts GAACCAGCAGATCCATAGCAAGGATCTCAGAG(+AAAAAA)GATCCTGGCTCATCCCAAGCAAAAGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606131114_zr4k ACGAATGCCTGCTTTTGCTTGGGATGAGCCAG(+AAAAAA)GATCCTCTGAGATCCTTGCTATGGATCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606131113_6c8m AGCAGGGGGAGGGGTGGTTAGATCTGAAAATG(+AAAAAA)GTACCAGTGACTTCTTATCTCTGGGTGACTT NGG 10 8 17 8 24 0 100 5 10
    240606131112_3fps TCCGGAAGTCACCCAGAGATAAGAAGTCACTG(+AAAAAA)GTACCATTTTCAGATCTAACCACCCCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606131110_gx71 ACTTGAATCTCACGTGGCAGGAGGGGCTATGG(+AAAAAA)GATCCGCTCGTCTTCCTCACTGCCGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240606131109_a4bk GGGGAGCTGTGCGGCAGTGAGGAAGACGAGCG(+AAAAAA)GATCCCATAGCCCCTCCTGCCACGTGAGATT NGG 10 8 17 8 24 0 100 5 10