Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131226_fj3b AGGTTCTCCTCTTCTCTGCCAGGGAAAGACAG(+AAAAAA)GATCCACACTGCTCTCCCAAACCCTTCCATT NGG 10 8 17 8 24 0 100 5 10
    240606131224_j712 GCCCCAATGGAAGGGTTTGGGAGAGCAGTGTG(+AAAAAA)GATCCTGTCTTTCCCTGGCAGAGAAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606131223_iugq ACCATTTGCTACCACTCATAGTAATGATAATG(+AAAAAA)GATCCGGGTAAGACTCATTGATGGAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606131221_32nn TTCCCCCAGCCTCCATCAATGAGTCTTACCCG(+AAAAAA)GATCCATTATCATTACTATGAGTGGTAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606131219_228k GAAATGATAATAAGATCTCCAAGAACATCTGG(+AAAAAA)GATCCAGAAATGACAATAGGAGAACTCATGA NGG 10 8 17 8 24 0 100 5 10
    240606131218_7mrn TGATTTCATGAGTTCTCCTATTGTCATTTCTG(+AAAAAA)GATCCCAGATGTTCTTGGAGATCTTATTATC NGG 10 8 17 8 24 0 100 5 10
    240606131216_3gdg TAATCACAACTCACAGGAGGCTTTGAGATTTG(+AAAAAA)GATCCTTAATAAAGCACTCCTTTTATGCAAA NGG 10 8 17 8 24 0 100 5 10
    240606131215_9ylp TGAGATTTGCATAAAAGGAGTGCTTTATTAAG(+AAAAAA)GATCCAAATCTCAAAGCCTCCTGTGAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606131213_7zie TTTTTCTTTAAAAAGCTTAAAGATTGGCATGG(+AAAAAA)GTACCGCTCTACTAATTATCTAAAACACAAC NGG 10 8 17 8 24 0 100 5 10
    240606131212_a4cw AAGCAGTTGTGTTTTAGATAATTAGTAGAGCG(+AAAAAA)GTACCCATGCCAATCTTTAAGCTTTTTAAAG NGG 10 8 17 8 24 0 100 5 10