Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131316_c9vb ACGAGTCATTGCTTCTGGTTTGTCCTAACAGG(+AAAAAA)GTACCTGACAGGAAATTGCTAGCTCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606131315_sqhy AGTTTCTGCCAGAGCTAGCAATTTCCTGTCAG(+AAAAAA)GTACCCTGTTAGGACAAACCAGAAGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240606131313_dngv GTAGGGTGTTCCTCCCAGTTCAGGTAGTGTTG(+AAAAAA)GATCCTGAGTTGTGGCTGGGATAACTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240606131312_0ph7 AATCCTGAAGAGTTATCCCAGCCACAACTCAG(+AAAAAA)GATCCAACACTACCTGAACTGGGAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606131311_9fmh TTACTCTCAAGGCTTCATGATCGACAATGAAG(+AAAAAA)GATCCTGCAGGAACGGAGGTGGTGGTGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606131309_lgoh CATCTGTGCCACCACCACCTCCGTTCCTGCAG(+AAAAAA)GATCCTTCATTGTCGATCATGAAGCCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606131307_hi3y AGACTGCAGCATTGGAGGCTCAGGGACCTTGG(+AAAAAA)GTACCTAAAGACAGACCTTGGGAAGCCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606131305_riro CTTCCCTTGGGCTTCCCAAGGTCTGTCTTTAG(+AAAAAA)GTACCCAAGGTCCCTGAGCCTCCAATGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606131304_tlrz AAATCATACCAAATGCACTCCACACTTTTTAG(+AAAAAA)GTACCTCCTTCCATCCACAGAAAGTAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606131302_std0 AACTGTGGCCTACTTTCTGTGGATGGAAGGAG(+AAAAAA)GTACCTAAAAAGTGTGGAGTGCATTTGGTAT NGG 10 8 17 8 24 0 100 5 10