Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131244_bw3s CTCCTCCTCCCTCCTCCCTCAGAAGTTCTGAG(+AAAAAA)GATCCTCGACAAGGTCACTGTCCACGACACT NGG 10 8 17 8 24 0 100 5 10
    240606131242_nx9v TCATTAGTGTCGTGGACAGTGACCTTGTCGAG(+AAAAAA)GATCCTCAGAACTTCTGAGGGAGGAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606131241_3vki GGTTGGGAATGTGGCAAATCATGGCCAGTCTG(+AAAAAA)GATCCCCTGGTCAGTGCTCTCTGTATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606131239_3e8d AGGAGCCCAGATACAGAGAGCACTGACCAGGG(+AAAAAA)GATCCAGACTGGCCATGATTTGCCACATTCC NGG 10 8 17 8 24 0 100 5 10
    240606131237_1431 TTAATTAGTCTAGCTACCCAGCTTGCTGTAGG(+AAAAAA)GATCCCTTTGTGCTTTTTTAAGGAGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606131235_4hsg TGAATTTTCTACTCCTTAAAAAAGCACAAAGG(+AAAAAA)GATCCCTACAGCAAGCTGGGTAGCTAGACTA NGG 10 8 17 8 24 0 100 5 10
    240606131234_4jp5 ACATCTGGGGTCAAGAGAAAGGGGAGTGAGAG(+AAAAAA)GATCCTCAAAGGATATCTCCTTTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606131232_jlao AGGAAGGAAGGGCAAAGGAGATATCCTTTGAG(+AAAAAA)GATCCTCTCACTCCCCTTTCTCTTGACCCCA NGG 10 8 17 8 24 0 100 5 10
    240606131231_fn4i AGTGTCAAGGTTTGGTGGCTGATTATGGGATG(+AAAAAA)GATCCCTGGGAGGGATATTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10
    240606131227_o4rv GGATGGACTATCCAGAGAATATCCCTCCCAGG(+AAAAAA)GATCCATCCCATAATCAGCCACCAAACCTTG NGG 10 8 17 8 24 0 100 5 10