Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131334_9ik9 GCGGCCCCAGCAACCGGGACGGAGGCTGCCGG(+AAAAAA)GATCCAGCCCGCGCCGCCAACGTGCGGTCGG NGG 10 8 17 8 24 0 100 5 10
    240606131332_zzsb GGGTTCCGACCGCACGTTGGCGGCGCGGGCTG(+AAAAAA)GATCCCGGCAGCCTCCGTCCCGGTTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606131330_rf77 ATGTTCCTGTATTTCTGCCCCTTGAGTGTTGG(+AAAAAA)GATCCCAGGCACAGACCTCACCACCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606131328_ito7 TCCTCTGGCTGGGTGGTGAGGTCTGTGCCTGG(+AAAAAA)GATCCCAACACTCAAGGGGCAGAAATACAGG NGG 10 8 17 8 24 0 100 5 10
    240606131327_czx3 AAGGAACCCACTATAATTAAGAGTAGCAAGAG(+AAAAAA)GTACCAGTAGGGGCTGTGTAATTCAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606131325_prq5 TCTTGTTTTATTGAATTACACAGCCCCTACTG(+AAAAAA)GTACCTCTTGCTACTCTTAATTATAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606131323_exye GGAGATCTAGTGTACTCTTCTGACCTCCAAAG(+AAAAAA)GTACCAGGTGAACATCCGGATGAATGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606131321_xsc1 ATTTTGCCTGCATTCATCCGGATGTTCACCTG(+AAAAAA)GTACCTTTGGAGGTCAGAAGAGTACACTAGA NGG 10 8 17 8 24 0 100 5 10
    240606131320_swy2 TCTAAAATGTATCATACATTAACGCAGGGCAG(+AAAAAA)GATCCATTAACATGGCTCCGCCTTTACAAGT NGG 10 8 17 8 24 0 100 5 10
    240606131318_iv5z CTGCCACTTGTAAAGGCGGAGCCATGTTAATG(+AAAAAA)GATCCTGCCCTGCGTTAATGTATGATACATT NGG 10 8 17 8 24 0 100 5 10