Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131408_oryr ACGGGCTAATCTGCTTGTCATGAAGCCCCAAG(+AAAAAA)GATCCCTCTCTGCTTCTCCACTGCTGACATT NGG 10 8 17 8 24 0 100 5 10
    240606131406_2619 GTTGCAATGTCAGCAGTGGAGAAGCAGAGAGG(+AAAAAA)GATCCTTGGGGCTTCATGACAAGCAGATTAG NGG 10 8 17 8 24 0 100 5 10
    240606131405_pvr6 TAAAAAGTCTTGCCTCTGAAGACCTTTTAAAG(+AAAAAA)GTACCATGAGTAGGAATTGGGAAGAATTGTT NGG 10 8 17 8 24 0 100 5 10
    240606131404_a6s5 ATTGTAACAATTCTTCCCAATTCCTACTCATG(+AAAAAA)GTACCTTTAAAAGGTCTTCAGAGGCAAGACT NGG 10 8 17 8 24 0 100 5 10
    240606131402_0pye TTTCTTAATGTTCTAATTATAATGAAAGTCGG(+AAAAAA)GATCCCATGTCACACTTTGGGAAACCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606131400_n3x5 CTAAATCAAAGGTTTCCCAAAGTGTGACATGG(+AAAAAA)GATCCCGACTTTCATTATAATTAGAACATTA NGG 10 8 17 8 24 0 100 5 10
    240606131359_2697 GTCTGTTTTACACTAGGTTCTAGAAGTAATTG(+AAAAAA)GATCCCAAAGTTCTTTGGCTATTATAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606131357_wxof GTCTATACTTTATAATAGCCAAAGAACTTTGG(+AAAAAA)GATCCAATTACTTCTAGAACCTAGTGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606131355_60jm GAAAACGAGACTCTATATGCCTTTTGACCATG(+AAAAAA)GATCCTGTGTTTGAATGACAGGCATTAATTT NGG 10 8 17 8 24 0 100 5 10
    240606131354_xfvh AGACCAAATTAATGCCTGTCATTCAAACACAG(+AAAAAA)GATCCATGGTCAAAAGGCATATAGAGTCTCG NGG 10 8 17 8 24 0 100 5 10