Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131456_yeja AAAAGAAAGAAAGAAACTGTTCTTTTCATTTG(+AAAAAA)GTACCATAGTGTGAAGAGCTGGGAGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240606131455_4oyl AACAGTCATTGCTCCCAGCTCTTCACACTATG(+AAAAAA)GTACCAAATGAAAAGAACAGTTTCTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606131454_qyvr CAAGCTCAGGTTCTCCCATCCAGGGGGAAGTG(+AAAAAA)GTACCCACGTTGACTAGAATGGGAAGATGTT NGG 10 8 17 8 24 0 100 5 10
    240606131452_e3wy TTGGGAACATCTTCCCATTCTAGTCAACGTGG(+AAAAAA)GTACCACTTCCCCCTGGATGGGAGAACCTGA NGG 10 8 17 8 24 0 100 5 10
    240606131451_1phv ATGTACTTCATGAGAAAATTCAACAGCAGCTG(+AAAAAA)GTACCGGATTCCAAGAAGGAAGGAAGCACAC NGG 10 8 17 8 24 0 100 5 10
    240606131449_t90r CACCCGTGTGCTTCCTTCCTTCTTGGAATCCG(+AAAAAA)GTACCAGCTGCTGTTGAATTTTCTCATGAAG NGG 10 8 17 8 24 0 100 5 10
    240606131447_v2r5 AAGTCACAGACTTTAAAAGTAGGTATGGGCGG(+AAAAAA)GATCCATCCAAGCCATCACTGGTCAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606131446_3zma GATGTCTCTCCTGACCAGTGATGGCTTGGATG(+AAAAAA)GATCCCGCCCATACCTACTTTTAAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606131444_ozjw GTCCCTGTGCTGATGGGGAGGAGGAGGGCAGG(+AAAAAA)GTACCGGGAGCAGGTAGCAGAAGGAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606131442_o6ku AAGGCAGAGGCTCCTTCTGCTACCTGCTCCCG(+AAAAAA)GTACCCTGCCCTCCTCCTCCCCATCAGCACA NGG 10 8 17 8 24 0 100 5 10