Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131542_pqeo AAAAGGACACATAGTATATAACTCCACTGTTG(+AAAAAA)GTACCAACAATATTTACTGCAGAAAACCACG NGG 10 8 17 8 24 0 100 5 10
    240606131540_e4oj GTTTCCGTGGTTTTCTGCAGTAAATATTGTTG(+AAAAAA)GTACCAACAGTGGAGTTATATACTATGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606131538_wp1q AAGTAGAAAATCATCTTTAAAAGACAGCCAAG(+AAAAAA)GTACCATCACATCTCCCTGAAAGTAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240606131537_6wg4 CTGTTCTGTCTTACTTTCAGGGAGATGTGATG(+AAAAAA)GTACCTTGGCTGTCTTTTAAAGATGATTTTC NGG 10 8 17 8 24 0 100 5 10
    240606131536_ps41 ATTGATTCCTACTTTATTATCTTAAAGACTAG(+AAAAAA)GATCCTGTTATTTTTCTTTAAATGTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606131535_ri21 AGTGCCTGTGAACATTTAAAGAAAAATAACAG(+AAAAAA)GATCCTAGTCTTTAAGATAATAAAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606131533_feel AAGGCCTACCTCCAGGGATGCAGCTCCTACAG(+AAAAAA)GATCCCAGAATGAAGTTGACTCGAATATTAC NGG 10 8 17 8 24 0 100 5 10
    240606131532_txjb CTGATGTAATATTCGAGTCAACTTCATTCTGG(+AAAAAA)GATCCTGTAGGAGCTGCATCCCTGGAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606131530_pcjx CCTGGGAGAGTTTTAGAATGTGTTTTTTCTGG(+AAAAAA)GTACCCTTCAAGTAAAAAACGCTTTGAATCT NGG 10 8 17 8 24 0 100 5 10
    240606131528_xc4g TCTGAAGATTCAAAGCGTTTTTTACTTGAAGG(+AAAAAA)GTACCCAGAAAAAACACATTCTAAAACTCTC NGG 10 8 17 8 24 0 100 5 10