Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131624_97mp TCCCTGTTCACCTCCTTACACAGAAAGATGTG(+AAAAAA)GTACCCAGGGTAGAATCACAGCTCCATACAG NGG 10 8 17 8 24 0 100 5 10
    240606131621_ivy5 GTACACTGTATGGAGCTGTGATTCTACCCTGG(+AAAAAA)GTACCACATCTTTCTGTGTAAGGAGGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240606131620_mrmk AGAGATCGCCTGCCTTGTGTCATGAGTGCTGG(+AAAAAA)GATCCATAAGCCACCATGCTTGGCTGATGTT NGG 10 8 17 8 24 0 100 5 10
    240606131618_6hb0 AGGGAAACATCAGCCAAGCATGGTGGCTTATG(+AAAAAA)GATCCCAGCACTCATGACACAAGGCAGGCGA NGG 10 8 17 8 24 0 100 5 10
    240606131617_phzc AAACTACATTTTTTTCTCTTGACTTCCTGTGG(+AAAAAA)GATCCCCATAGCAGTCTTTCAGATAAAGATT NGG 10 8 17 8 24 0 100 5 10
    240606131615_ht4v ATCTTAATCTTTATCTGAAAGACTGCTATGGG(+AAAAAA)GATCCCACAGGAAGTCAAGAGAAAAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240606131613_bmwi AGAAATTCAGGGCTGAAGAATAGACCCAGGCG(+AAAAAA)GATCCAGGCACAGCCAGATCCACCCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606131611_p1j2 TCTTAGCTACAGGGTGGATCTGGCTGTGCCTG(+AAAAAA)GATCCGCCTGGGTCTATTCTTCAGCCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606131610_s88p TTGAAGTCCCACTGTTGGCAGTTTTGGCAGTG(+AAAAAA)GTACCTAACTCATCGTGTTTGCATTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606131609_73zz AACTTAACAAGAATGCAAACACGATGAGTTAG(+AAAAAA)GTACCACTGCCAAAACTGCCAACAGTGGGAC NGG 10 8 17 8 24 0 100 5 10