Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131723_s5aj CGCTGCTTGTATATGAAAATAAAAGGCATACG(+AAAAAA)GTACCCCTGAGACAAAAGCCGAGGGGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606131721_mkoq GAATAACACCCCCCTCGGCTTTTGTCTCAGGG(+AAAAAA)GTACCGTATGCCTTTTATTTTCATATACAAG NGG 10 8 17 8 24 0 100 5 10
    240606131720_7spt CTGTGAGGTCAAGGCATCGAGCGGCATAGTAG(+AAAAAA)GATCCTGTCTCAAGCACACCGGCTAGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606131719_q6jj AGCTACCACTCTAGCCGGTGTGCTTGAGACAG(+AAAAAA)GATCCTACTATGCCGCTCGATGCCTTGACCT NGG 10 8 17 8 24 0 100 5 10
    240606131717_shmz CAGGAGACCAATCCTGCCAGGTGGGCAAGCTG(+AAAAAA)GATCCGGGTAACAGCTATTTGCAAAGAACAA NGG 10 8 17 8 24 0 100 5 10
    240606131715_912a GTTGGTTGTTCTTTGCAAATAGCTGTTACCCG(+AAAAAA)GATCCAGCTTGCCCACCTGGCAGGATTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606131713_fe1w GCACTTCTTGCTCTAGCATCTTCTTCAAGTGG(+AAAAAA)GATCCAGCAAAGGAGGCTGAGATGTCTAAGG NGG 10 8 17 8 24 0 100 5 10
    240606131712_82dw CATGACCTTAGACATCTCAGCCTCCTTTGCTG(+AAAAAA)GATCCCACTTGAAGAAGATGCTAGAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606131711_17wf AAAACTAGGTTGGAATTGAAACATTTTGTAAG(+AAAAAA)GTACCTTAATCCTTGGTAATTATTAGCATAT NGG 10 8 17 8 24 0 100 5 10
    240606131709_nf4y TAATCATATGCTAATAATTACCAAGGATTAAG(+AAAAAA)GTACCTTACAAAATGTTTCAATTCCAACCTA NGG 10 8 17 8 24 0 100 5 10