Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131739_lmdt ACCTCGTCTTCCCCTCCTTCAGGTTAGCCTGG(+AAAAAA)GTACCAGTAGCACGTCTCCTAGTGGCCGGCA NGG 10 8 17 8 24 0 100 5 10
    240606131737_u17q AAGGGTGCCGGCCACTAGGAGACGTGCTACTG(+AAAAAA)GTACCCAGGCTAACCTGAAGGAGGGGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240606131736_93uc TCAGCGGAATGGCGGATGTGCTTAACAGTCAG(+AAAAAA)GATCCTACTGACAAGTGCTGACCATTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240606131735_6vha ACCCAGTAGAAATGGTCAGCACTTGTCAGTAG(+AAAAAA)GATCCTGACTGTTAAGCACATCCGCCATTCC NGG 10 8 17 8 24 0 100 5 10
    240606131734_4pqn GAACATGGTCCAAGGTATTTATAAACAACTAG(+AAAAAA)GTACCCCAGGACAGGTTGAGAGACCACATCT NGG 10 8 17 8 24 0 100 5 10
    240606131731_252x CCAAAAGATGTGGTCTCTCAACCTGTCCTGGG(+AAAAAA)GTACCTAGTTGTTTATAAATACCTTGGACCA NGG 10 8 17 8 24 0 100 5 10
    240606131729_c5bl AGATGTTGCATGCACCAACTGAGCTATCATTG(+AAAAAA)GATCCAAGTGGTCAGCGCTGAAATCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606131727_qezp ACGGAAGGAGAGATTTCAGCGCTGACCACTTG(+AAAAAA)GATCCAATGATAGCTCAGTTGGTGCATGCAA NGG 10 8 17 8 24 0 100 5 10
    240606131726_i15z CCCTTCCTGAGGACTGTAACTCCAGCTCTGGG(+AAAAAA)GATCCAGTGTCCTCTCGTGCCCTCCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606131724_qr7h GTGCATGCTCTGGAGGGCACGAGAGGACACTG(+AAAAAA)GATCCCCAGAGCTGGAGTTACAGTCCTCAGG NGG 10 8 17 8 24 0 100 5 10