Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131824_4skp ATGAAATGAACAATTTTCTAGACAGATACCTG(+AAAAAA)GTACCAAAGTTAAATCAGGATCAGATAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606131823_k1t5 TTAGATTTTTATCTGATCCTGATTTAACTTTG(+AAAAAA)GTACCAGGTATCTGTCTAGAAAATTGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240606131821_esu5 ATGATCTGAAAATACTTACATATACCTGGAAG(+AAAAAA)GTACCTAAGTCATCCAACTGAATGCCAACTG NGG 10 8 17 8 24 0 100 5 10
    240606131820_cwks ATGCCCAGTTGGCATTCAGTTGGATGACTTAG(+AAAAAA)GTACCTTCCAGGTATATGTAAGTATTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606131819_5ngm AAAAGGCTGGAAAACAATTTTCCAAGCAAATG(+AAAAAA)GTACCAAGAAACAAGCTAGAGTAGCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240606131817_s94d ATATTAGGATGGCTACTCTAGCTTGTTTCTTG(+AAAAAA)GTACCATTTGCTTGGAAAATTGTTTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606131816_eb9j ATGAAGTGGACACTTTTCTAGACAGATACTAG(+AAAAAA)GTACCAAAGTTAAATCAGGATCAGATTAACG NGG 10 8 17 8 24 0 100 5 10
    240606131815_gnl9 TAAATCGTTAATCTGATCCTGATTTAACTTTG(+AAAAAA)GTACCTAGTATCTGTCTAGAAAAGTGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606131813_z7qv TGCTCCTAGTCACCCATTGGATGCAGCACAGG(+AAAAAA)GTACCCAATGAAGGAGCTAAAGAAAGTACCT NGG 10 8 17 8 24 0 100 5 10
    240606131811_rcim TCTTTAGGTACTTTCTTTAGCTCCTTCATTGG(+AAAAAA)GTACCCTGTGCTGCATCCAATGGGTGACTAG NGG 10 8 17 8 24 0 100 5 10