Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131841_fs4f CATCAAGCAACTCTGAGTCTCTCTGGCTTCTG(+AAAAAA)GATCCTGCTACAATATATGTGGGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240606131839_2chk CCCTTTGTTCCTGCCCACATATATTGTAGCAG(+AAAAAA)GATCCAGAAGCCAGAGAGACTCAGAGTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606131837_9016 ACGGCCAAGCCATCCAAAACTAAGGGAAGTGG(+AAAAAA)GATCCCTAAGAGGTGAACCCTTAAGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606131835_i5fb TGGGGACAACACTTAAGGGTTCACCTCTTAGG(+AAAAAA)GATCCCACTTCCCTTAGTTTTGGATGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606131834_38i8 TTTATATTCCAATTATGCCAACAAAGGGCAAG(+AAAAAA)GTACCAGGAAAAGCATTAAACTATATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606131832_9m58 GTGTTAAAAAATATAGTTTAATGCTTTTCCTG(+AAAAAA)GTACCTTGCCCTTTGTTGGCATAATTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240606131830_7jru CTGTCAGTATAGTTCAAGTTTACCAGTAGAGG(+AAAAAA)GTACCTGAGAGGAACAACCACCACAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606131829_6itp TGAGGCAGCTCTGTGGTGGTTGTTCCTCTCAG(+AAAAAA)GTACCCTCTACTGGTAAACTTGAACTATACT NGG 10 8 17 8 24 0 100 5 10
    240606131827_exby AAAAGGCTGGAAAACAATTTTCCAAGCAAATG(+AAAAAA)GTACCAAGAAACAAGCTAGAGTAGCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240606131826_ryl1 ATATTAGGATGGCTACTCTAGCTTGTTTCTTG(+AAAAAA)GTACCATTTGCTTGGAAAATTGTTTTCCAGC NGG 10 8 17 8 24 0 100 5 10