Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131810_m2bm CATAGTGAGGAAATGAAATGATGCACTTAATG(+AAAAAA)GTACCATCTCACTGTCAAGATAAAATACCCA NGG 10 8 17 8 24 0 100 5 10
    240606131809_3a0v GCAGCTGGGTATTTTATCTTGACAGTGAGATG(+AAAAAA)GTACCATTAAGTGCATCATTTCATTTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240606131807_bso3 ATTGTTGAAAGTCAAACTTATTGTGAGTTAAG(+AAAAAA)GATCCATAAAATTCTTCTGTTGCAGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606131806_ba7m AGTATCCCTAACTGCAACAGAAGAATTTTATG(+AAAAAA)GATCCTTAACTCACAATAAGTTTGACTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606131805_c4yd AATCCACTCAGGACCACACAGTAACACATGTG(+AAAAAA)GATCCTGGCACCCCCACTTCTGCCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240606131803_yovq CTGGAAGTTGGAGGCAGAAGTGGGGGTGCCAG(+AAAAAA)GATCCACATGTGTTACTGTGTGGTCCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240606131801_4p2b CTCTACAAAGATGTGATGCTGGAGATCTTCAG(+AAAAAA)GATCCTCACTACTATAGGCTACACCTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606131800_189p ATGTCTTCCCAGGTGTAGCCTATAGTAGTGAG(+AAAAAA)GATCCTGAAGATCTCCAGCATCACATCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606131758_szeq AATAGCCACATGGGTACTGAGAATTAATCCTG(+AAAAAA)GATCCTCTGAAAATGCCAGGTAATCAATGAG NGG 10 8 17 8 24 0 100 5 10
    240606131757_vj9c GACGGCTCATTGATTACCTGGCATTTTCAGAG(+AAAAAA)GATCCAGGATTAATTCTCAGTACCCATGTGG NGG 10 8 17 8 24 0 100 5 10