Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131653_jop3 AATAAAAGTAAGAAAATATGCTCAATGCTGAG(+AAAAAA)GTACCTTAAAAAAACAAAAGTTTATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240606131652_xzcs TAAAATATTTTATAAACTTTTGTTTTTTTAAG(+AAAAAA)GTACCTCAGCATTGAGCATATTTTCTTACTT NGG 10 8 17 8 24 0 100 5 10
    240606131651_2ru1 TGCTCAACCTTATTTATATCACTGACTGGCTG(+AAAAAA)GATCCAAAGTCACACCTCCACATATTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240606131649_wu1m TTTATTCACTAATATGTGGAGGTGTGACTTTG(+AAAAAA)GATCCAGCCAGTCAGTGATATAAATAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606131648_jxjf ACTGTGGGTGCTTGAGGAGGCCAGAGGCATAG(+AAAAAA)GATCCCTCTGGAGCATGAATTACAAGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606131646_id7k CTGACAGCCACTTGTAATTCATGCTCCAGAGG(+AAAAAA)GATCCTATGCCTCTGGCCTCCTCAAGCACCC NGG 10 8 17 8 24 0 100 5 10
    240606131645_f89p CCAACAATAAAAATAAATCTTAAAGGGGGAGG(+AAAAAA)GTACCAACAGTGCCTGACACTACATGAACTC NGG 10 8 17 8 24 0 100 5 10
    240606131643_mvcj AGGAAGAGTTCATGTAGTGTCAGGCACTGTTG(+AAAAAA)GTACCCTCCCCCTTTAAGATTTATTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240606131641_p8y9 TTTAGGACTGACACCCTGAGTTTAGTCCCTGG(+AAAAAA)GATCCTCGTGGTGGGAGAGAGAACTGACTTC NGG 10 8 17 8 24 0 100 5 10
    240606131639_ca54 GTGTGGAAGTCAGTTCTCTCTCCCACCACGAG(+AAAAAA)GATCCCAGGGACTAAACTCAGGGTGTCAGTC NGG 10 8 17 8 24 0 100 5 10