Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131440_jjqk GAGCTGACCTTCCCAGGAACCTCACAGTTCCG(+AAAAAA)GATCCTTTGTCTCAGTAGATACAGCTTAGCC NGG 10 8 17 8 24 0 100 5 10
    240606131439_vkak CATGAGGCTAAGCTGTATCTACTGAGACAAAG(+AAAAAA)GATCCGGAACTGTGAGGTTCCTGGGAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606131437_uj0j CTGCTAGGAAGCACACAAGTTTCATCACAGGG(+AAAAAA)GTACCCAAGTGCCTCATACCATTTATGAATT NGG 10 8 17 8 24 0 100 5 10
    240606131435_7scm ATCTAAATTCATAAATGGTATGAGGCACTTGG(+AAAAAA)GTACCCCTGTGATGAAACTTGTGTGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606131434_8mxh CTGCTAGGAAGCACACAAGTTTCATCACAGGG(+AAAAAA)GTACCCAAGTGCCTCATACCATTTATGAATT NGG 10 8 17 8 24 0 100 5 10
    240606131432_damu ATCTAAATTCATAAATGGTATGAGGCACTTGG(+AAAAAA)GTACCCCTGTGATGAAACTTGTGTGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606131431_cffk CAGGTCCTGTAGTTGTCTTCTTCTCATGTTTG(+AAAAAA)GTACCGCTAAGCTTTTCAAACTGTCTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606131429_h53t AGGGTTGAAAAGACAGTTTGAAAAGCTTAGCG(+AAAAAA)GTACCAAACATGAGAAGAAGACAACTACAGG NGG 10 8 17 8 24 0 100 5 10
    240606131428_547o TTGTGTGTATTTATTAGAGCATTTGCATACTG(+AAAAAA)GTACCTTTTATTAAAAGGGTTGATTTGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606131427_9q0q GTAAAACACCAAATCAACCCTTTTAATAAAAG(+AAAAAA)GTACCAGTATGCAAATGCTCTAATAAATACA NGG 10 8 17 8 24 0 100 5 10