Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131352_q7x8 TTATAGGCCTTGAAACATAAAAGGCAATCCAG(+AAAAAA)GTACCAACACAGCGCACACACGACACAAATG NGG 10 8 17 8 24 0 100 5 10
    240606131351_7c90 TCTTTCATTTGTGTCGTGTGTGCGCTGTGTTG(+AAAAAA)GTACCTGGATTGCCTTTTATGTTTCAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606131350_bmbh TGCTGATTTAAGCAGAACACAAAACAAATATG(+AAAAAA)GATCCTAGGGCTTACTTCCACCAGTACCAGC NGG 10 8 17 8 24 0 100 5 10
    240606131347_eyzh TCCACGCTGGTACTGGTGGAAGTAAGCCCTAG(+AAAAAA)GATCCATATTTGTTTTGTGTTCTGCTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606131345_43s4 AAACTAGGAAAGATGGGAGGCAGAGACAGGAG(+AAAAAA)GATCCCTGGGTTCAAAGGTCTACAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606131342_tiuf GGGGTTTCTCTTTGTAGACCTTTGAACCCAGG(+AAAAAA)GATCCTCCTGTCTCTGCCTCCCATCTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606131341_g0k4 ACAGTAAAAGTGATGTCGCTTGAGAGAAGGTG(+AAAAAA)GTACCCTCGGTGCCGGCTTGCACACTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606131338_0msh TACTTCAGCTAGTGTGCAAGCCGGCACCGAGG(+AAAAAA)GTACCACCTTCTCTCAAGCGACATCACTTTT NGG 10 8 17 8 24 0 100 5 10
    240606131337_07ry TTGGGGTCCCTCAGCACTTTGCCCTGATTTCG(+AAAAAA)GATCCTGAGTAACAGATACCACTGAATTGCT NGG 10 8 17 8 24 0 100 5 10
    240606131336_7noa GTTACAGCAATTCAGTGGTATCTGTTACTCAG(+AAAAAA)GATCCGAAATCAGGGCAAAGTGCTGAGGGAC NGG 10 8 17 8 24 0 100 5 10