Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131005_4v2g TGGGCTCTATGAATGATGCAACACATTAGATG(+AAAAAA)GTACCTCCAAGGCTGGCTTCTACCATGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606131004_mslx AGGGTAACTCATGGTAGAAGCCAGCCTTGGAG(+AAAAAA)GTACCATCTAATGTGTTGCATCATTCATAGA NGG 10 8 17 8 24 0 100 5 10
    240606131002_yrit GAGGCTCCAGCCAAGATTGACAGATCCAGGTG(+AAAAAA)GATCCTGATCCATTATTTAGCTATCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606131001_5fcg TCACTGGAAGAGATAGCTAAATAATGGATCAG(+AAAAAA)GATCCACCTGGATCTGTCAATCTTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606130959_k3qj AAAGGGGCAGTGATGCTGAGCTGATCTAGGAG(+AAAAAA)GATCCCTGGACCGTGCTCCATAGCATCAAAC NGG 10 8 17 8 24 0 100 5 10
    240606130957_uzxe ATCTCGTTTGATGCTATGGAGCACGGTCCAGG(+AAAAAA)GATCCTCCTAGATCAGCTCAGCATCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240606130956_az22 TCTTGATGATAATGGAGAGAGGACCCTGAAAG(+AAAAAA)GTACCTCCAGTTAGCTTAAGCCAGAGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240606130954_vqk5 AAAGAGGCGACTCTGGCTTAAGCTAACTGGAG(+AAAAAA)GTACCTTTCAGGGTCCTCTCTCCATTATCAT NGG 10 8 17 8 24 0 100 5 10
    240606130953_dlqp GGCAGCTCAGCCCTTTGCCTTTTCAGGCTATG(+AAAAAA)GATCCCATGTAGCCTGGGAGTTGGCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606130952_ctzw TGACTGAGGAGGCCAACTCCCAGGCTACATGG(+AAAAAA)GATCCATAGCCTGAAAAGGCAAAGGGCTGAG NGG 10 8 17 8 24 0 100 5 10