Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130555_a0lh CTTTATGGTCAAGAGATTATTATGACACAGGG(+AAAAAA)GTACCATTAGCGTAACAGCCCTCCAGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606130554_ar1r TGAGCACTCTCTGGAGGGCTGTTACGCTAATG(+AAAAAA)GTACCCCTGTGTCATAATAATCTCTTGACCA NGG 10 8 17 8 24 0 100 5 10
    240606130552_uq8a GGCACATGGGTCAGAACAAAGTTGCATTATGG(+AAAAAA)GTACCAGCGATCCAATTAATTAATATTTATA NGG 10 8 17 8 24 0 100 5 10
    240606130551_6er0 CCAGCTATAAATATTAATTAATTGGATCGCTG(+AAAAAA)GTACCCATAATGCAACTTTGTTCTGACCCAT NGG 10 8 17 8 24 0 100 5 10
    240606130549_uz5g GTCATGGGCACTGTGGCTGAAGTGGCTGCTTG(+AAAAAA)GTACCAGCCGTCTCAGTAGCAGGAGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606130548_ylo0 CTTGTGGCTCACTCCTGCTACTGAGACGGCTG(+AAAAAA)GTACCAAGCAGCCACTTCAGCCACAGTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606130546_jdb7 AACATTCCAGGCCGTTCGGAAGGCATCACTGG(+AAAAAA)GTACCAGACTTGAAACCAGTCTTCAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240606130545_xwiv ACACTTGTTACTGAAGACTGGTTTCAAGTCTG(+AAAAAA)GTACCCAGTGATGCCTTCCGAACGGCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606130544_fre1 CCGTGGGATTATAGGAAAACCAGGGCCTCTTG(+AAAAAA)GTACCCTGTCCTCAGAGTGTGTGACAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606130542_p10v TTACAGTGGCTGTCACACACTCTGAGGACAGG(+AAAAAA)GTACCAAGAGGCCCTGGTTTTCCTATAATCC NGG 10 8 17 8 24 0 100 5 10