Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130541_orpl GCACCTCCGAAGCTCTTAGTAATGTTGTGCTG(+AAAAAA)GATCCTGTAAGTTCGCTCTGCAGTCACTCGC NGG 10 8 17 8 24 0 100 5 10
    240606130540_97p5 GGTTTGCGAGTGACTGCAGAGCGAACTTACAG(+AAAAAA)GATCCAGCACAACATTACTAAGAGCTTCGGA NGG 10 8 17 8 24 0 100 5 10
    240606130538_5kxh TGATGCTCCACAAACTGTCTCATTAATCCATG(+AAAAAA)GATCCTAAAACAAATTCTCTTTGTGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240606130537_zd62 CACACACAATACACAAAGAGAATTTGTTTTAG(+AAAAAA)GATCCATGGATTAATGAGACAGTTTGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606130535_51pz TCTTCCTTTTGAAGCTAGGCTGTGCTGTGTGG(+AAAAAA)GATCCAGTGGTCTGTTCTTTACTGGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606130533_jgut AACACATACCTCCAGTAAAGAACAGACCACTG(+AAAAAA)GATCCCACACAGCACAGCCTAGCTTCAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606130532_oo8f AGTAAAACCTTTAAACATGGTGACTATCTTCG(+AAAAAA)GATCCCAAGGAGAATATTAACAGGCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240606130530_uvwg GAGTTCCTGGTGCCTGTTAATATTCTCCTTGG(+AAAAAA)GATCCGAAGATAGTCACCATGTTTAAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606130529_0d7u GTAAAAAAAAAAAAAAAGGCAGATGGCAGATG(+AAAAAA)GATCCTGGAGCTTTTTCTCTCTTTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606130527_4ubt CTGGGGAAAAAAAAAGAGAGAAAAAGCTCCAG(+AAAAAA)GATCCATCTGCCATCTGCCTTTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10