Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130432_ut8v GTGCACAGTTCTGTGAGGCTTTGGGGCAAGGG(+AAAAAA)GTACCAAGAGCCCAAGAGAGCCTTTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606130431_4h2y TTTAGAACAAGAAAGGCTCTCTTGGGCTCTTG(+AAAAAA)GTACCCCTTGCCCCAAAGCCTCACAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240606130430_motk TAGGGAGGGATGTCAAGGCCAAGAGTCATACG(+AAAAAA)GTACCCAGTAACTGCTGGTGTAGCTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606130428_32my TGCCTCCCAAGAGCTACACCAGCAGTTACTGG(+AAAAAA)GTACCGTATGACTCTTGGCCTTGACATCCCT NGG 10 8 17 8 24 0 100 5 10
    240606130426_kljj CCAACAGGGAGGGACCAAGGGCAGCCACCAGG(+AAAAAA)GATCCTATCTCACCTCCGATGCCTGCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606130425_6zia CCTGGACACAGCAGGCATCGGAGGTGAGATAG(+AAAAAA)GATCCCTGGTGGCTGCCCTTGGTCCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606130423_te2j CATGGGCTAGTTTCCATTAGGCCCTCCATGGG(+AAAAAA)GATCCTAAAGAGGGAGAGGGAATGAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606130421_h223 CTGCTTTTCTCTCATTCCCTCTCCCTCTTTAG(+AAAAAA)GATCCCCATGGAGGGCCTAATGGAAACTAGC NGG 10 8 17 8 24 0 100 5 10
    240606130420_qqxr GCTCATAATTATCTGTAACTCCAATTTCAGGG(+AAAAAA)GATCCAACACCCTCATAAAGACACACATGCA NGG 10 8 17 8 24 0 100 5 10
    240606130419_s7gk TTGCCTGCATGTGTGTCTTTATGAGGGTGTTG(+AAAAAA)GATCCCCTGAAATTGGAGTTACAGATAATTA NGG 10 8 17 8 24 0 100 5 10