Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122232_bdy5 TGTGGACCCCTACTGGGACGTGCTTGGTTTGG(+AAAAAA)GTACCTTCCTCGGTCCCACTGGCCGATTCTC NGG 10 8 17 8 24 0 100 5 10
    240606122230_zbd9 AAGTTGAGAATCGGCCAGTGGGACCGAGGAAG(+AAAAAA)GTACCCAAACCAAGCACGTCCCAGTAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606122229_cokc CCACCTTGAAATCCCAAAGAAAGCAGTTCTTG(+AAAAAA)GTACCATCTATTATGGTTCTACCATTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606122228_jeff CCCCTTTTTTAATGGTAGAACCATAATAGATG(+AAAAAA)GTACCAAGAACTGCTTTCTTTGGGATTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606122226_k208 TCTCAATGCAGAATTACTGTGTGCAGGGGGAG(+AAAAAA)GTACCAGGTCCCTTTCATTTGGGCTCAACTC NGG 10 8 17 8 24 0 100 5 10
    240606122224_t824 TGTTTGAGTTGAGCCCAAATGAAAGGGACCTG(+AAAAAA)GTACCTCCCCCTGCACACAGTAATTCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240606122222_zse9 ATATATGATGAAGGTGGGTCAGCTCTGGTTGG(+AAAAAA)GATCCGAGAAGTGACACAGCTGGAGAATGTC NGG 10 8 17 8 24 0 100 5 10
    240606122221_8gmk TAAGTGACATTCTCCAGCTGTGTCACTTCTCG(+AAAAAA)GATCCCAACCAGAGCTGACCCACCTTCATCA NGG 10 8 17 8 24 0 100 5 10
    240606122219_x9gt AGTCAACAGGGACAGCAGAATGACCACTAATG(+AAAAAA)GATCCTTAATCTTCTTCTAGTAGCTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606122218_82qu ACTTCCCCCAAAGCTACTAGAAGAAGATTAAG(+AAAAAA)GATCCATTAGTGGTCATTCTGCTGTCCCTGT NGG 10 8 17 8 24 0 100 5 10