Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122335_8xj2 TCTTGCTGAAATAAAGGTAAAGTCCACATTTG(+AAAAAA)GATCCTTTGCTCCTGCACCACTTTTCCATTG NGG 10 8 17 8 24 0 100 5 10
    240606122333_rvx7 CCAGCCAATGGAAAAGTGGTGCAGGAGCAAAG(+AAAAAA)GATCCAAATGTGGACTTTACCTTTATTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606122331_oyqy TTTTTCTCTGAAATTCCTTGTCTCCGCCTCCG(+AAAAAA)GATCCAGGTCAGCAGCAGCCCCCAGAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606122329_gbbu CAGCCACAGCTCTGGGGGCTGCTGCTGACCTG(+AAAAAA)GATCCGGAGGCGGAGACAAGGAATTTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606122327_qmwv TCAGCCTGAACCATTTCTCATCTGTTTCCCAG(+AAAAAA)GATCCAAAATTGTACCTGCCTTTCAATAACT NGG 10 8 17 8 24 0 100 5 10
    240606122326_yt67 GGAGGAGTTATTGAAAGGCAGGTACAATTTTG(+AAAAAA)GATCCTGGGAAACAGATGAGAAATGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606122324_zm63 GAGGTCTTAAGATCCCATGGAGGGTCCTGTGG(+AAAAAA)GTACCTTGTGGGGGTCCGCAGACTCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240606122323_mfnq CCTTGGGCACAGAGTCTGCGGACCCCCACAAG(+AAAAAA)GTACCCACAGGACCCTCCATGGGATCTTAAG NGG 10 8 17 8 24 0 100 5 10
    240606122320_yzqc GGATGGACCATGTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606122319_q2xc GGTGTCAGCATTTGGAAGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10