Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122428_g6a6 GGGAGCACAGGGAGGAGGCGTGTGAGGTTGAG(+AAAAAA)GATCCGTCTGCTGTGGATTCTTCTGTTTGCA NGG 10 8 17 8 24 0 100 5 10
    240606122427_rmus AGCGCTGCAAACAGAAGAATCCACAGCAGACG(+AAAAAA)GATCCTCAACCTCACACGCCTCCTCCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606122424_g9wr GCTTCTCCCGGGAGGGCTGCCCAGAACCAGGG(+AAAAAA)GATCCTCCTCCTCCACAAACCCGAGGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606122423_lk1p GTGTCCCCTTCCTCGGGTTTGTGGAGGAGGAG(+AAAAAA)GATCCCCTGGTTCTGGGCAGCCCTCCCGGGA NGG 10 8 17 8 24 0 100 5 10
    240606122421_zgbd AGCTAGAGTACCCCTTCCCTCCCCACCACTGG(+AAAAAA)GATCCTTGATGGTACAGAGCAATTTTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606122420_jrwf CTGCACAAGCAAAATTGCTCTGTACCATCAAG(+AAAAAA)GATCCCAGTGGTGGGGAGGGAAGGGGTACTC NGG 10 8 17 8 24 0 100 5 10
    240606122418_6ksl TTTTTTTTTTTTTTTTTTTTTTTTAAACTTGG(+AAAAAA)GATCCCTAGGGCAGCTTGCACAGGAAATCCT NGG 10 8 17 8 24 0 100 5 10
    240606122415_x9nt ACCAAAGGATTTCCTGTGCAAGCTGCCCTAGG(+AAAAAA)GATCCCAAGTTTAAAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606122414_rs77 GCTAACAACTATCTGTAACTCTACTCTAAAGG(+AAAAAA)GATCCGATGATCTCTGGCTCTACTGGGCACT NGG 10 8 17 8 24 0 100 5 10
    240606122413_7mib CATAAAGTGCCCAGTAGAGCCAGAGATCATCG(+AAAAAA)GATCCCTTTAGAGTAGAGTTACAGATAGTTG NGG 10 8 17 8 24 0 100 5 10