Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122515_qbvd CACTCTGTGGACCAGGCTGGCCTCAAACTCAG(+AAAAAA)GATCCTCCTGAGTGCTGGGATTAAAGGCGTA NGG 10 8 17 8 24 0 100 5 10
    240606122513_rst9 TGGTGTACGCCTTTAATCCCAGCACTCAGGAG(+AAAAAA)GATCCTGAGTTTGAGGCCAGCCTGGTCCACA NGG 10 8 17 8 24 0 100 5 10
    240606122512_7sqd GTAAGGACCGACTTGCGGGTAGCATAGTCTTG(+AAAAAA)GTACCAGATGATGTTTTTAGGGACATCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606122511_0qp2 TGTTTCTTTGATGTCCCTAAAAACATCATCTG(+AAAAAA)GTACCAAGACTATGCTACCCGCAAGTCGGTC NGG 10 8 17 8 24 0 100 5 10
    240606122509_ujot GCAGGGGAGCTCAGAGCTTCAGCCCTGCCTAG(+AAAAAA)GTACCACAGTGAAAACAGGCAATCCGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606122507_8e97 GGTGGGGGGACGGATTGCCTGTTTTCACTGTG(+AAAAAA)GTACCTAGGCAGGGCTGAAGCTCTGAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606122506_oj29 GAGTATGTTGTAGCTATCTTCAGAGGGCATGG(+AAAAAA)GATCCCATGGTGAGCCACCATGTGGGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606122503_x531 ATTCCCAGCACCCACATGGTGGCTCACCATGG(+AAAAAA)GATCCCATGCCCTCTGAAGATAGCTACAACA NGG 10 8 17 8 24 0 100 5 10
    240606122502_qlsz GCAACTCTTAAATAAAGCAAATATACAGACTG(+AAAAAA)GATCCGGCTGAGACAAGCTGGGTAATCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606122500_0d8h CAAATGTCTGATTACCCAGCTTGTCTCAGCCG(+AAAAAA)GATCCAGTCTGTATATTTGCTTTATTTAAGA NGG 10 8 17 8 24 0 100 5 10