Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122458_lr5k AGATCATGTCTCCACTTACTGTCACTCCTAAG(+AAAAAA)GATCCCCTGAAGGTCTTGGAGCTTCACATCC NGG 10 8 17 8 24 0 100 5 10
    240606122456_4nmo TCCAGGGATGTGAAGCTCCAAGACCTTCAGGG(+AAAAAA)GATCCTTAGGAGTGACAGTAAGTGGAGACAT NGG 10 8 17 8 24 0 100 5 10
    240606122454_p6r0 CGATCTATGACAATGTTTGGAACTCTCTTTTG(+AAAAAA)GATCCACAAAGCTCCTGCAATCAGCTCCAAT NGG 10 8 17 8 24 0 100 5 10
    240606122453_8ws3 GGGTTATTGGAGCTGATTGCAGGAGCTTTGTG(+AAAAAA)GATCCAAAAGAGAGTTCCAAACATTGTCATA NGG 10 8 17 8 24 0 100 5 10
    240606122452_0jyh ACTACTCTTGGCTTATATTAGACAGTAGAGTG(+AAAAAA)GTACCCCTACCCCATGGTACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240606122450_njkf GCATGTGTGTGTGTGTGTACCATGGGGTAGGG(+AAAAAA)GTACCACTCTACTGTCTAATATAAGCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606122449_43kk TACATTCAAGGACAGCCTGGGCTACAGGATAG(+AAAAAA)GATCCTATCTAAAAACAAAACAAAAATAACC NGG 10 8 17 8 24 0 100 5 10
    240606122447_2t4z TTGAGGGTTATTTTTGTTTTGTTTTTAGATAG(+AAAAAA)GATCCTATCCTGTAGCCCAGGCTGTCCTTGA NGG 10 8 17 8 24 0 100 5 10
    240606122445_3rw0 TGGTTTTGTGGGTGATTTTTCCATATTCCTAG(+AAAAAA)GATCCTAGTAGTTTAAGTGTGTAAATGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606122444_gh5c GAAGTCAGACATTTACACACTTAAACTACTAG(+AAAAAA)GATCCTAGGAATATGGAAAAATCACCCACAA NGG 10 8 17 8 24 0 100 5 10