Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122545_f2eg TAATAAGTCTCGGTGTCATCATTTAACTGGAG(+AAAAAA)GATCCAGAAAGTCCCACTACAATGTATTGCA NGG 10 8 17 8 24 0 100 5 10
    240606122544_jomw CTATCTGCAATACATTGTAGTGGGACTTTCTG(+AAAAAA)GATCCTCCAGTTAAATGATGACACCGAGACT NGG 10 8 17 8 24 0 100 5 10
    240606122543_45wf GACAGATCCAGTTCAAAATCCTAGAGGCAGTG(+AAAAAA)GATCCTACAAGAGACTTGTGATTTTTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606122542_6dua AGGGAAGAAAAAAAATCACAAGTCTCTTGTAG(+AAAAAA)GATCCACTGCCTCTAGGATTTTGAACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606122540_evq6 CCAGTGCTAGCACTTGCTTACTGCCTCAGCTG(+AAAAAA)GATCCTAACACTAGCCCTTCAAGATTAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606122539_tjf5 ATCCTGGCTTAATCTTGAAGGGCTAGTGTTAG(+AAAAAA)GATCCAGCTGAGGCAGTAAGCAAGTGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240606122537_hnsr TATTTTTTAAAAATTATTTTTGTGGACATGGG(+AAAAAA)GATCCCGTGACGGCACCTACTTTTGGGAGTC NGG 10 8 17 8 24 0 100 5 10
    240606122535_o9ah CTACAGACTCCCAAAAGTAGGTGCCGTCACGG(+AAAAAA)GATCCCCATGTCCACAAAAATAATTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606122533_14dt TTTTCTACAGTCCTCCCCTGTTGTACCTAGGG(+AAAAAA)GTACCAAGAAAACATAGGAATATCTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240606122532_iaic TTATTTAGTGCAGATATTCCTATGTTTTCTTG(+AAAAAA)GTACCCCTAGGTACAACAGGGGAGGACTGTA NGG 10 8 17 8 24 0 100 5 10