Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122411_gzom AAAATTGAAACTATAAACCAAGTACCCCACTG(+AAAAAA)GTACCCATCTTGTCTAAGCCCCTCTAACCTC NGG 10 8 17 8 24 0 100 5 10
    240606122409_58uz TGAGGGAGGTTAGAGGGGCTTAGACAAGATGG(+AAAAAA)GTACCAGTGGGGTACTTGGTTTATAGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606122408_yvsp GACTAGCTCATGTTAGCTCAGGCTCTGCTCAG(+AAAAAA)GATCCCAGCCAGCCCTGCTCTGCTCTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606122406_cq1b AAGACAGAGCAGAGCAGAGCAGGGCTGGCTGG(+AAAAAA)GATCCTGAGCAGAGCCTGAGCTAACATGAGC NGG 10 8 17 8 24 0 100 5 10
    240606122404_k30m CAGGGAAATACCCAATGTTGTTCTGCTCCCTG(+AAAAAA)GATCCCCGCAACTGTTTTCCCTCCCACTCTG NGG 10 8 17 8 24 0 100 5 10
    240606122401_ezmt TGCTCCAGAGTGGGAGGGAAAACAGTTGCGGG(+AAAAAA)GATCCAGGGAGCAGAACAACATTGGGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606122359_tafe TTGCTGTCCTTGGTCTTACACAGAGAACCCAG(+AAAAAA)GTACCAGGCCACCTCCTTACTCAGAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606122357_4d5a GCAGACTCTCTTCTGAGTAAGGAGGTGGCCTG(+AAAAAA)GTACCTGGGTTCTCTGTGTAAGACCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606122354_zc2p AGCTCACAGGCACCTCCAACTCAAGCTCCGGG(+AAAAAA)GATCCAACCTCCTTGCTGGTTTCTTTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606122353_b0yo CCTGATGCCCAAAGAAACCAGCAAGGAGGTTG(+AAAAAA)GATCCCCGGAGCTTGAGTTGGAGGTGCCTGT NGG 10 8 17 8 24 0 100 5 10