Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120907_hjlz GCTCATAGAACACGGCGCATGATGGGTAAGAG(+AAAAAA)GATCCAGACTACCCAGTGTGTCCCAGGTATT NGG 10 8 17 8 24 0 100 5 10
    240606120905_72rk CTGGGAATACCTGGGACACACTGGGTAGTCTG(+AAAAAA)GATCCTCTTACCCATCATGCGCCGTGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240606120904_oqh1 TTAGTGTCCTTTAGTCTTCGCACTTGTCTAAG(+AAAAAA)GATCCACTTTTAGGGCTTGCCCACATGTGAT NGG 10 8 17 8 24 0 100 5 10
    240606120903_efl3 ATAGAATCACATGTGGGCAAGCCCTAAAAGTG(+AAAAAA)GATCCTTAGACAAGTGCGAAGACTAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606120902_wn1h GCTGTCTTCAGACCCATTAGAAGAGGGCATCG(+AAAAAA)GATCCCATTATAGATGGTTATAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606120900_s05d ACCACATGGTGGCTTATAACCATCTATAATGG(+AAAAAA)GATCCGATGCCCTCTTCTAATGGGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606120857_ovkg GGTCAGGAGGACCCAGCGCCTACTGCACCCAG(+AAAAAA)GATCCATTAGCACTGTAACAACGAACACCTG NGG 10 8 17 8 24 0 100 5 10
    240606120856_g329 CCACTCAGGTGTTCGTTGTTACAGTGCTAATG(+AAAAAA)GATCCTGGGTGCAGTAGGCGCTGGGTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606120854_izhm GCAGAACTGAATAAACACAGGGTGTGAGGATG(+AAAAAA)GATCCTGCCTGGCTGTGCATCCCTGACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606120853_jyic GCAGCTGGAGTCAGGGATGCACAGCCAGGCAG(+AAAAAA)GATCCATCCTCACACCCTGTGTTTATTCAGT NGG 10 8 17 8 24 0 100 5 10