Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120802_4hk2 GGGTAGGAATTGAAGACATGGTCACACCTCAG(+AAAAAA)GTACCAGTTTCTGCTCTGCCAGGCGCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606120801_cwvl AGTGTAGAAAGCGCCTGGCAGAGCAGAAACTG(+AAAAAA)GTACCTGAGGTGTGACCATGTCTTCAATTCC NGG 10 8 17 8 24 0 100 5 10
    240606120759_51v4 GTACTCCGGGCTGCCCCAGAAACCTGCCTTTG(+AAAAAA)GATCCATCTTGTCTGTTTAGAGAGAGCGGAG NGG 10 8 17 8 24 0 100 5 10
    240606120757_gnk5 GGGCACTCCGCTCTCTCTAAACAGACAAGATG(+AAAAAA)GATCCAAAGGCAGGTTTCTGGGGCAGCCCGG NGG 10 8 17 8 24 0 100 5 10
    240606120756_e3ke GGATGTCCTTCCAGGTCAATAATCTTAGCAGG(+AAAAAA)GTACCTTCCTCAGAATGGCCAGAGCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606120754_g8re CTCCTTCTCCAGCTCTGGCCATTCTGAGGAAG(+AAAAAA)GTACCCTGCTAAGATTATTGACCTGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606120752_fimx CACCAGAAAGAGATCATGATGTGCCAGCCAGG(+AAAAAA)GATCCTGCGACCCAGATCTTCTCTCCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606120751_aw64 AACTGTCTCTGGAGAGAAGATCTGGGTCGCAG(+AAAAAA)GATCCCTGGCTGGCACATCATGATCTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606120750_kibq GGGGGGGGGGGCTGGGTTTTTGGAATCTGTAG(+AAAAAA)GATCCATAGCACAATCATCTACCACACACCT NGG 10 8 17 8 24 0 100 5 10
    240606120749_l1nc GAAGTAGGTGTGTGGTAGATGATTGTGCTATG(+AAAAAA)GATCCTACAGATTCCAAAAACCCAGCCCCCC NGG 10 8 17 8 24 0 100 5 10