Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120658_xczv GTGTTCTCCCTCCATCCACTTAGGCCCAGATG(+AAAAAA)GTACCTAGTAAACAAGCCATAAACACATGGG NGG 10 8 17 8 24 0 100 5 10
    240606120657_mjbr CAAGTCCCATGTGTTTATGGCTTGTTTACTAG(+AAAAAA)GTACCATCTGGGCCTAAGTGGATGGAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606120655_4mzv TTTTGATTTGGGTCCAACCAAAACCTTCCTTG(+AAAAAA)GATCCATCTCTTGCTCACTTACACAGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606120653_3ksy CCATGGAGGCCTGTGTAAGTGAGCAAGAGATG(+AAAAAA)GATCCAAGGAAGGTTTTGGTTGGACCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606120651_84ru GCTGTGAGGAAGGTCCTGTGCCGACACAGTGG(+AAAAAA)GTACCTGGCAAGGGTAAGAGGAGAGCCATCA NGG 10 8 17 8 24 0 100 5 10
    240606120650_8s8j GCTGGTGATGGCTCTCCTCTTACCCTTGCCAG(+AAAAAA)GTACCCACTGTGTCGGCACAGGACCTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606120648_t5ke GGGTCTCTGGGTGATGAGGTCAGAGTCTCTGG(+AAAAAA)GATCCCCTGAAATCCCGGAGAAGAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606120646_ish0 TTCTTCCCAGGCTCTTCTCCGGGATTTCAGGG(+AAAAAA)GATCCCAGAGACTCTGACCTCATCACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606120644_y1yl TCACAACCATCTTTGTAACTCCAGTTTCAAGG(+AAAAAA)GATCCAACCCCCTCTCCTGACCTCAAACACC NGG 10 8 17 8 24 0 100 5 10
    240606120643_52fo AGTCTGGTGTTTGAGGTCAGGAGAGGGGGTTG(+AAAAAA)GATCCCTTGAAACTGGAGTTACAAAGATGGT NGG 10 8 17 8 24 0 100 5 10