Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120624_6ex9 CAGAACCCAGACTCTTACCCTTACCCAAAATG(+AAAAAA)GATCCAGTTCCACTGCCTCCCTCTCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606120622_3coe CAGTGAGGAGGGAGAGGGAGGCAGTGGAACTG(+AAAAAA)GATCCATTTTGGGTAAGGGTAAGAGTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606120620_zkcg TATCCTTTCTGTTTTGGGCTCTACAGCCTCTG(+AAAAAA)GATCCACTGGGCCTTGGCAACCCTTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606120619_qr7r GAGCCAAGAACAAGGGTTGCCAAGGCCCAGTG(+AAAAAA)GATCCAGAGGCTGTAGAGCCCAAAACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606120617_sf29 CCTCCAGAGCTCACCTTCCACGCAGTGATGAG(+AAAAAA)GATCCTGTCAGGGACCCTACCTCACAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606120616_a0df AGCTGACTGCTGTGAGGTAGGGTCCCTGACAG(+AAAAAA)GATCCTCATCACTGCGTGGAAGGTGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606120615_lbbx GCAGGGTGGCAGTCACCTCCCCTGTCCACGTG(+AAAAAA)GATCCATCCCTGCCATTGTGTGAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606120613_j7mg CTATCCCTCTGACTCACACAATGGCAGGGATG(+AAAAAA)GATCCACGTGGACAGGGGAGGTGACTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606120612_0v72 ACGGAGAACCAAGGAAGACAATGTTTCATCCG(+AAAAAA)GATCCAGAGGCGCATCAAATTCAACAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606120610_fih5 GTGGAGCTGCTGTTGAATTTGATGCGCCTCTG(+AAAAAA)GATCCGGATGAAACATTGTCTTCCTTGGTTC NGG 10 8 17 8 24 0 100 5 10