Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120551_krzv AATAGTGAGCGGGGAAAGGAGAATAGGAAGTG(+AAAAAA)GATCCTTCCAGAACCTACCTCTCTCTGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606120550_a4dt GTTGAGCTTCAGAGAGAGGTAGGTTCTGGAAG(+AAAAAA)GATCCACTTCCTATTCTCCTTTCCCCGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606120548_deya CCTCTCTTTACGTGAGATACTGTGGCGTGCTG(+AAAAAA)GTACCATCCAAGCCTCGGCCCGGTGTCTATC NGG 10 8 17 8 24 0 100 5 10
    240606120547_ou7u GAGGGGATAGACACCGGGCCGAGGCTTGGATG(+AAAAAA)GTACCAGCACGCCACAGTATCTCACGTAAAG NGG 10 8 17 8 24 0 100 5 10
    240606120544_57kz GCACGAGGCAACTGCAGAAGTACACTCCCAGG(+AAAAAA)GTACCCTTTTCTCCCTGAAACTTCTTACGGG NGG 10 8 17 8 24 0 100 5 10
    240606120542_ihrc GTGTGCCCGTAAGAAGTTTCAGGGAGAAAAGG(+AAAAAA)GTACCCTGGGAGTGTACTTCTGCAGTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606120541_41ar TACTGTTCCAGGTGTGGAGGGGGAATGGAGAG(+AAAAAA)GTACCTGGTAGGCATCCACAGGGGACATGAT NGG 10 8 17 8 24 0 100 5 10
    240606120539_bt45 CTGAGATCATGTCCCCTGTGGATGCCTACCAG(+AAAAAA)GTACCTCTCCATTCCCCCTCCACACCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606120537_kng0 GGGAGAGAGTTACTGCTGCCAGCTTTCCTCTG(+AAAAAA)GTACCCGCTATAGACTGGCATCCAGAGATGG NGG 10 8 17 8 24 0 100 5 10
    240606120535_jqvp GGCACCCATCTCTGGATGCCAGTCTATAGCGG(+AAAAAA)GTACCAGAGGAAAGCTGGCAGCAGTAACTCT NGG 10 8 17 8 24 0 100 5 10