Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120502_1mho AACTGTTCTTGTCCTTGTCACTCAGGGCAGAG(+AAAAAA)GATCCTGGGCATACTGAGGAGACCATTGCTA NGG 10 8 17 8 24 0 100 5 10
    240606120500_31hl GACAATAGCAATGGTCTCCTCAGTATGCCCAG(+AAAAAA)GATCCTCTGCCCTGAGTGACAAGGACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606120459_obcd AACTGTTCTTGTCCTTGTCACTCAGGGCAGAG(+AAAAAA)GATCCTGGGCATACTGAGGAGACCATTGCTA NGG 10 8 17 8 24 0 100 5 10
    240606120456_do0t GACAATAGCAATGGTCTCCTCAGTATGCCCAG(+AAAAAA)GATCCTCTGCCCTGAGTGACAAGGACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606120454_miif AAGAATGTGTGTCATGGTAGAGGGGTTCCCAG(+AAAAAA)GATCCTCTCCTGCCAGATGCTTGGCTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606120453_iew9 GACCTGCAGCAGCCAAGCATCTGGCAGGAGAG(+AAAAAA)GATCCTGGGAACCCCTCTACCATGACACACA NGG 10 8 17 8 24 0 100 5 10
    240606120450_sb91 CACCTACCCCGATGCCCGAGTTCAACTCCCAG(+AAAAAA)GATCCACTTGGTGGAAGGAGACCACTGATCC NGG 10 8 17 8 24 0 100 5 10
    240606120449_i4o0 TGTGGGGATCAGTGGTCTCCTTCCACCAAGTG(+AAAAAA)GATCCTGGGAGTTGAACTCGGGCATCGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606120448_igvq CCAGTTGGTACACTGTTTTCTGAAAGAGGTGG(+AAAAAA)GATCCAAAAGAGCTGTTTCTAGCTGCACTCC NGG 10 8 17 8 24 0 100 5 10
    240606120446_yv3l TGCTTGGAGTGCAGCTAGAAACAGCTCTTTTG(+AAAAAA)GATCCCACCTCTTTCAGAAAACAGTGTACCA NGG 10 8 17 8 24 0 100 5 10