Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120413_953r GCAGTCCCCACGCAGGGTGGCTTCCTGTCGAG(+AAAAAA)GATCCAGTGGGCCCAGCGTTGGTCCACCCAC NGG 10 8 17 8 24 0 100 5 10
    240606120410_yh2p ACTGAGTGGGTGGACCAACGCTGGGCCCACTG(+AAAAAA)GATCCTCGACAGGAAGCCACCCTGCGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606120408_blly GGCAGGTATCAGAGGTGAAGGTATTCTGGTGG(+AAAAAA)GATCCCCCTCCCAAGGGACCTTGGCTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606120406_l5f5 CTAGGACTCCAGCCAAGGTCCCTTGGGAGGGG(+AAAAAA)GATCCCACCAGAATACCTTCACCTCTGATAC NGG 10 8 17 8 24 0 100 5 10
    240606120404_jtw4 GCTATGTGATATTCCACAGGTGTGTGGTCCGG(+AAAAAA)GATCCCTCAGGTAGAGAAAAGGAAACCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606120402_3spc CGTCAGCAAGGTTTCCTTTTCTCTACCTGAGG(+AAAAAA)GATCCCGGACCACACACCTGTGGAATATCAC NGG 10 8 17 8 24 0 100 5 10
    240606120400_0s8z GGGGCTCACTGCTCCACTAGGCTGGCTGCAGG(+AAAAAA)GATCCTACTGCCTCTACCTTCCCAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606120359_ghgo TAATTCCAGCACTGGGAAGGTAGAGGCAGTAG(+AAAAAA)GATCCCTGCAGCCAGCCTAGTGGAGCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240606120358_xph5 AGGACGCTAGAGGGCGCTCTAGGACAGCGTCG(+AAAAAA)GATCCTGTCTCTGGCAACCCCTTGCAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606120356_xens CCCGTGGGCTTGCAAGGGGTTGCCAGAGACAG(+AAAAAA)GATCCGACGCTGTCCTAGAGCGCCCTCTAGC NGG 10 8 17 8 24 0 100 5 10