Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120355_u4dy GGCATCTGACTCAAGGAATAGTCTGGAATAAG(+AAAAAA)GATCCGGGTCTGTTTACCCTGGTAGCCTCCG NGG 10 8 17 8 24 0 100 5 10
    240606120353_quxz TCAGCCGGAGGCTACCAGGGTAAACAGACCCG(+AAAAAA)GATCCTTATTCCAGACTATTCCTTGAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606120350_0mtj AAGCCATCCTAGGAAGACCAAGTGATCCCATG(+AAAAAA)GATCCTGTACACCTCTCCTGGAGGAGCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606120349_625o TGTACCTGGGCTCCTCCAGGAGAGGTGTACAG(+AAAAAA)GATCCATGGGATCACTTGGTCTTCCTAGGAT NGG 10 8 17 8 24 0 100 5 10
    240606120348_c75k TGCTGAGACACAGAATGAGTTTTATGGCAAAG(+AAAAAA)GATCCTTAATCCCCAGAGACGCTTTGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606120347_qfu5 CACCACAAGCCAAAGCGTCTCTGGGGATTAAG(+AAAAAA)GATCCTTTGCCATAAAACTCATTCTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606120344_lbkx TTTCTCTGGAGTCCTTCTTGGAGAAAACCAGG(+AAAAAA)GATCCAGGTTCATCGCCGAGGCCCTCGGAGC NGG 10 8 17 8 24 0 100 5 10
    240606120343_vbqr GCCCCGCTCCGAGGGCCTCGGCGATGAACCTG(+AAAAAA)GATCCCTGGTTTTCTCCAAGAAGGACTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606120341_aatd CATTGTGCCTCTGAGACATCCTTAGGGCTGGG(+AAAAAA)GTACCCAGCTCTCGAAGACACCATGCCATGA NGG 10 8 17 8 24 0 100 5 10
    240606120339_kpix AGAAGTCATGGCATGGTGTCTTCGAGAGCTGG(+AAAAAA)GTACCCCAGCCCTAAGGATGTCTCAGAGGCA NGG 10 8 17 8 24 0 100 5 10