Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120305_zjl6 CTCCCCATGGATTCATGTGCACCCATCTGGAG(+AAAAAA)GTACCAGATTCCTCCATGGCAGATCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606120303_nvvz TGTTCCCAACAGATCTGCCATGGAGGAATCTG(+AAAAAA)GTACCTCCAGATGGGTGCACATGAATCCATG NGG 10 8 17 8 24 0 100 5 10
    240606120301_k34g CCCCATGCTACTGATAACACAGTGATCACCAG(+AAAAAA)GATCCAAACTGGCTTCAGGTTTCTAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240606120300_o4pb GACGCTTTTGTTAGAAACCTGAAGCCAGTTTG(+AAAAAA)GATCCTGGTGATCACTGTGTTATCAGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240606120259_pgy5 ACTCCATAGATCTCAGTAATTCTGGAAACAAG(+AAAAAA)GTACCATGACATGCAGGTCATCATTGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606120258_auhu TTTGGTTTTCCAATGATGACCTGCATGTCATG(+AAAAAA)GTACCTTGTTTCCAGAATTACTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240606120257_66uc AAAAGGATATACCTTCTTCTCAGCACCTCATG(+AAAAAA)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240606120255_soq3 TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+AAAAAA)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240606120254_iw5w ACGAAATGGACAAATTTCTGGACAGATACCAG(+AAAAAA)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240606120253_fvyj TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+AAAAAA)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10