Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120320_y1i4 GTTCCACTTAGTCAGGAAGTCTCCAGCCTATG(+AAAAAA)GATCCTGTGCCACCCCCTGCCCCCAACGCCC NGG 10 8 17 8 24 0 100 5 10
    240606120319_oz5j TGTTTGGGCGTTGGGGGCAGGGGGTGGCACAG(+AAAAAA)GATCCATAGGCTGGAGACTTCCTGACTAAGT NGG 10 8 17 8 24 0 100 5 10
    240606120318_pf3c TGAGATGAGTCCACCTCATCTGTAGCCTAGAG(+AAAAAA)GTACCTCAGTGAGTTGGGAAAGTCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606120316_oo9b TAAGTGGCCTCTGACTTTCCCAACTCACTGAG(+AAAAAA)GTACCTCTAGGCTACAGATGAGGTGGACTCA NGG 10 8 17 8 24 0 100 5 10
    240606120315_plee TCGCTGGGGCTGCCCCATAGGACACTGGGGTG(+AAAAAA)GTACCATGGAGAGACAGGGGATAAAACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606120313_8760 TTTGGATCAGTTTTATCCCCTGTCTCTCCATG(+AAAAAA)GTACCACCCCAGTGTCCTATGGGGCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606120311_dke4 GCTCAAAATGTCTCTGGCTGTATTGATCTGAG(+AAAAAA)GATCCTCCCCTCTTTTGAACCATTCCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606120309_2a95 GGTGATGGAAGGAATGGTTCAAAAGAGGGGAG(+AAAAAA)GATCCTCAGATCAATACAGCCAGAGACATTT NGG 10 8 17 8 24 0 100 5 10
    240606120308_hvi6 CAAGTCTGTGCCTCAGCACAGCTAGAAGTCAG(+AAAAAA)GATCCAAGGAGGCCTAAAATCGGCAATGACA NGG 10 8 17 8 24 0 100 5 10
    240606120306_515d GAGTCTGTCATTGCCGATTTTAGGCCTCCTTG(+AAAAAA)GATCCTGACTTCTAGCTGTGCTGAGGCACAG NGG 10 8 17 8 24 0 100 5 10