Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120445_nsg1 TGCCAGTCTGTGTCTGTCTAATCAGAAGGATG(+AAAAAA)GATCCCTGTACCTAACATTCCATATCACTAC NGG 10 8 17 8 24 0 100 5 10
    240606120443_a2hb TCAGTGTAGTGATATGGAATGTTAGGTACAGG(+AAAAAA)GATCCATCCTTCTGATTAGACAGACACAGAC NGG 10 8 17 8 24 0 100 5 10
    240606120442_o2bt AAGGGCTATTCAAGCTCCCAAGAAAATCTGTG(+AAAAAA)GATCCATGGGTTACAGATTAAAGTTCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240606120439_i4p4 ACCCAGTTTGGAACTTTAATCTGTAACCCATG(+AAAAAA)GATCCACAGATTTTCTTGGGAGCTTGAATAG NGG 10 8 17 8 24 0 100 5 10
    240606120438_u7vm GACATTTTTACCATCTGCTCTCTTCAGTATCG(+AAAAAA)GATCCGTATTGAGAAAAGAGAAGCACCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606120437_ozdj GCAGCGTCTGGTGCTTCTCTTTTCTCAATACG(+AAAAAA)GATCCGATACTGAAGAGAGCAGATGGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606120435_8b6b GGGTCTGAGTTTGCTTTCCGTTGCTGTGGTGG(+AAAAAA)GTACCACGACTGGAGCAACATGGGGGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606120434_q8ie TAAAGCCTTTCCCCCATGTTGCTCCAGTCGTG(+AAAAAA)GTACCCACCACAGCAACGGAAAGCAAACTCA NGG 10 8 17 8 24 0 100 5 10
    240606120432_n0ps CTGTTGTGTCTAGCTTGTAGGTGTGCTCTGGG(+AAAAAA)GATCCCAAGTCAGGTCATCAGACTTGCACTG NGG 10 8 17 8 24 0 100 5 10
    240606120430_hsmq GCTTGCAGTGCAAGTCTGATGACCTGACTTGG(+AAAAAA)GATCCCCAGAGCACACCTACAAGCTAGACAC NGG 10 8 17 8 24 0 100 5 10