Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120607_26tt CTCTGGAGTGAAAACATAAAACCTTGTCCCTG(+AAAAAA)GTACCGTGAAGCCATGCAAGAGACGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606120606_vj1y TTTTCTCACCTCGTCTCTTGCATGGCTTCACG(+AAAAAA)GTACCAGGGACAAGGTTTTATGTTTTCACTC NGG 10 8 17 8 24 0 100 5 10
    240606120604_6u48 ACGTATCCACAGTGCAATGTTGGCTCAGCCAG(+AAAAAA)GATCCACCTTGCATACATACTAAAGAATGAC NGG 10 8 17 8 24 0 100 5 10
    240606120603_xn4c GATCAGTCATTCTTTAGTATGTATGCAAGGTG(+AAAAAA)GATCCTGGCTGAGCCAACATTGCACTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606120601_838v ATGTAGTGCCTTTTTTGTGTTCTGAGGGAGTG(+AAAAAA)GTACCCTGAGGTCTCGTCGTGTAGTTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240606120600_3lj9 TTAACTCTAAAACTACACGACGAGACCTCAGG(+AAAAAA)GTACCACTCCCTCAGAACACAAAAAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606120558_pyxv TGCCAAGAGCTGGTAAGCGACACAACCCATGG(+AAAAAA)GTACCAGGGATTCTGGCTGACACAAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606120555_cb0v TCAGGTTCACCTTGTGTCAGCCAGAATCCCTG(+AAAAAA)GTACCCATGGGTTGTGTCGCTTACCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606120554_k8ta TACTCACGGTCTCTGTTTTCTCACTCTCTGAG(+AAAAAA)GTACCACAGGCAGGCAGCCCTTGAGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606120552_gygf CATTGGCTGAGCTCAAGGGCTGCCTGCCTGTG(+AAAAAA)GTACCTCAGAGAGTGAGAAAACAGAGACCGT NGG 10 8 17 8 24 0 100 5 10