Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120716_2xar CAAGTAAATGGCCTTGAAGTGGCCATGCCTTG(+AAAAAA)GTACCGAGCAGTCAAACTGATGAACACGGCA NGG 10 8 17 8 24 0 100 5 10
    240606120715_vu8v ACAAATGCCGTGTTCATCAGTTTGACTGCTCG(+AAAAAA)GTACCAAGGCATGGCCACTTCAAGGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240606120713_k54m CTATCTAGATGGACCTGTATAATGTCTGCCTG(+AAAAAA)GTACCTGAGATCTAGCCTCTGCACTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606120712_hszo ATTTGCCACTAAGTGCAGAGGCTAGATCTCAG(+AAAAAA)GTACCAGGCAGACATTATACAGGTCCATCTA NGG 10 8 17 8 24 0 100 5 10
    240606120710_npiz GGTCAACCTTGCCAGGCTTCTCCTAGCCCTTG(+AAAAAA)GATCCACATCTTCCAGGGAGAGAGGCTGACA NGG 10 8 17 8 24 0 100 5 10
    240606120708_44kh ATGTGTGTCAGCCTCTCTCCCTGGAAGATGTG(+AAAAAA)GATCCAAGGGCTAGGAGAAGCCTGGCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606120705_i2fx TATGGGCTACAGAGCCTAGCTGAGCCCCCAGG(+AAAAAA)GATCCCAGAAACTGTCTTTAGTCTCTTATGT NGG 10 8 17 8 24 0 100 5 10
    240606120703_l9t3 CAATAACATAAGAGACTAAAGACAGTTTCTGG(+AAAAAA)GATCCCTGGGGGCTCAGCTAGGCTCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606120702_ydcp GAAATTGTGGACAAACCCCTGGAGAGATGCAG(+AAAAAA)GTACCCGGGCAGCATCCTCCGGGGCTCTGCG NGG 10 8 17 8 24 0 100 5 10
    240606120659_i7uh ACATACGCAGAGCCCCGGAGGATGCTGCCCGG(+AAAAAA)GTACCTGCATCTCTCCAGGGGTTTGTCCACA NGG 10 8 17 8 24 0 100 5 10