Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115816_1wwf TGGGAAGCCATAGGAGGGTGCTTAAAAAAAGG(+AAAAAA)GATCCAGTTTTATGGTGATGTGGAGGGGCTA NGG 10 8 17 8 24 0 100 5 10
    240606115814_aujt GTGAAAGGTGCCAGGAGTGGGAGGCTCCCAAG(+AAAAAA)GATCCAAATGTGCGTGGTGCAGGCGCATGCC NGG 10 8 17 8 24 0 100 5 10
    240606115812_478t GACTTGGCATGCGCCTGCACCACGCACATTTG(+AAAAAA)GATCCTTGGGAGCCTCCCACTCCTGGCACCT NGG 10 8 17 8 24 0 100 5 10
    240606115810_76d2 TCATAACGGAACAAGAACAGCTTGCCTACCTG(+AAAAAA)GATCCATGCTGCAGGCCTCTCAGGTCTTACC NGG 10 8 17 8 24 0 100 5 10
    240606115809_hfwe AGGTAGGTAAGACCTGAGAGGCCTGCAGCATG(+AAAAAA)GATCCAGGTAGGCAAGCTGTTCTTGTTCCGT NGG 10 8 17 8 24 0 100 5 10
    240606115808_2khg AGCTGCTGTGTGGGTGCTAGGAATTTAGCTTG(+AAAAAA)GATCCTCTGGAAGAGCAGCCAGTGCTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606115806_kirf GTGGTTAAAGAGCACTGGCTGCTCTTCCAGAG(+AAAAAA)GATCCAAGCTAAATTCCTAGCACCCACACAG NGG 10 8 17 8 24 0 100 5 10
    240606115804_ln8o TCAGATGCTAGCCTTTGGGGTTTCTGTGGAGG(+AAAAAA)GATCCATACCATAGCCAAAAATAAAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606115803_6qk4 TCTAGAATTCTTTTATTTTTGGCTATGGTATG(+AAAAAA)GATCCCTCCACAGAAACCCCAAAGGCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240606115801_o5cn ATGCATCTGTCTGTACACCACATTTGTGCCTG(+AAAAAA)GTACCTGGTGCCTGGTGCCAATGGAGACCAG NGG 10 8 17 8 24 0 100 5 10